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Cells were then plated for experiments at 2.5 10 5 cells ml 1 in RPMI 1640 containing 25 mM HEPES, 1% penicillin/streptomycin, 10% FBS (dialyzed used for metabolite extraction experiments) and 20 ng ml 1 recombinant mouse macrophage colony stimulating factor (R&D Systems)

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3.2 Non-pharmacological treatments of PDs The non-pharmacological treatments of PDs include various approaches like physical, occupational, speech, and nutritional therapy and exercise (58, 59)

In certain embodiments, the amount of filler, diluent, or mannitol is from about 1 mg to about 150 mg