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Abnormal glucose metabolism, especially hyperglycemia, leads to oxidative stress, microvascular damage, vascular tone and endothelial damage, as well as platelet aggregation and embolism (37, 38)

Using a novel and validated fluorescent KRAS Y137W variant amenable to stopped-flow spectroscopy, we characterized the kinetics of KRAS G12C fluorescence changes upon addition of ARS-853 or AMG 510, noting that ARS-853 addition at 5C elicited both a rapid first phase (attributed to binding, yielding a K d of 36.0 0.7 M), and a second, slower pH-dependent phase taken to represent covalent ligation

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Then, 1 H, 13 C, Dept 135, and Dept 90 experiments were conducted on the final products with 16, 400, 200, and 200 accumulations, respectively

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