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CIP: Chromatin Immunoprecipitation

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175,176 Mammalian TORC1 is implicated in regulating the size, morphology, and synaptic plasticity of neurons and maintaining energy balance within the central nervous system

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The cell concentration was determined by manual counting in a haemocytometer, and 10,000 cells were plated into each well of a 48-well plate and the medium mixed with nRFP- or nGFP-encoding lentivirus (LVP360-R and LVP360-G, GenTarget) to achieve a multiplicity of infection (MOI) of 1030, then replaced after 12 h and the solution was changed after 72 h