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A 2013 study found that high vitamin B levels were linked with a subsequent cancer diagnosis, which was similarly found in a 2020 study that showed that high levels of serum B12 could be linked with the development of solid cancers (tumours)

Materials and methods Cell culture, regents and plasmids All cell lines (HeLa, MDA-MB-231, and HEK293T) were obtained from the American Type Culture Collection and were maintained in Dulbeccos modified Eagle medium (DMEM, Gibco, 8121298) supplemented with 10% fetal bovine serum (FBS, Gibco, 2176398) and 1% penicillinstreptomycin at 37 C with 5% CO 2

Studies have shown that between 1996 and 2016, the number of people with Parkinsons disease (PD) doubled (13)

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The DCFH-DA probe, obtained from Solarbio's ROS detection kit (Solarbio, Beijing, China), was diluted 2000-fold for the assay