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8 Future directions and research gaps Despite the increasing body of evidence that elucidates the synergistic interactions of CIT, leucine, and physical exercise on the enhancement of muscle and vascular health in the aging population, several methodological limitations persist within the extant literature

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After reaching 95% confluence, the cells were induced to differentiate using DMEM with 10% FBS, 0.5 g ml 1 insulin, 1 nM T3, 0.125 mM indomethacin, 2 g ml 1 dexamethasone, 0.5 M IBMX and P/S (day 0)

In addition to more classical nutrients, metabolomics analysis may provide insights into dietary factors that might be deficient or present in insufficient levels in a diet that could contribute to disease

Nausea reduction from B12: The B12 compound directly supports tolerability in the early weeks when the body is adjusting to the medication