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Density gradient centrifugation was performed, and individual islets with high purity were hand-picked under a dissecting microscope

Interestingly, polyphenols molecules are a class of molecules that can have a positive effect on cancer onset and development, by affecting several distinct metabolic pathways, hence maybe having a synergistic effect: cell cycle, apoptosis, cell division, energy metabolism, DNA maintenance, are just some of the mechanisms of action of polyphenols in our metabolism [25-29]

[15] This is exactly the biological logic that made the local MGF splice variant interesting in the first place: nature appears to use a transient, compartmentalized IGF-1 signal for repair, precisely to avoid the metabolic disturbances that broad, sustained IGF-1 receptor activation would cause including effects on glucose homeostasis

doi: 10.3389/fcell.2021.616551 54 LiXGNiuCLuPWanHWJinWDWangCXet al

Next, cDNA was synthesized using 1 g of RNA with a PrimeScript RT Reagent Kit with gDNA Eraser following a set procedure (50 C, 15 min