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Combinatory antibiotic treatment protects against experimental acute pancreatitis by suppressing gut bacterial translocation to pancreas and inhibiting NLRP3 inflammasome pathway

After the introduction of the New Generation Sequencing (NGS) technology [30], which relies on platforms for high-throughput, massively parallel sequencing that can analyse many genes at once, it was possible to identify the forms of PFIC, from PFIC1 to PFIC13 [4, 8, 11, 33,34,35,36,37]

Louis, MO, USA), tetrahydrofuran (C 4 H 8 O, 99.9%,Cornaredo MI, Italy), donepezil hydrochloride (C 24 H 30 ClNO 3 , 98% (HPLC), Sigma Aldrich, Lu Wan Qu, Shanghai, China), streptozotocin (C 8 H 15 N 3 O 7 , 98% (HPLC), 265.22 g/mol, Sigma Aldrich, Saint Louis, MO, USA), citrate buffer (0.1 M, pH 4.5, Biodiagnostic, Egypt), O-acetylcholine iodide (C 7 H 16 INO 2 , 99%, 273.12 g/mol, BDH chemicals, Poole, Dorset, England), 5,5-Dithio-bis(2-nitrobenzoic acid) (C 14 H 8 N 2 O 8 S 2 , 98%, 396.36 g/mol, Corsham, England), ELISA kit for brain derived neurotrophic factor (BDNF) (Elabscience, Wuhan, Hubei, China), Commercial kits from biodiagnostic, Egypt were used for the measurement of MDA, GSH and SOD

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Other disorders must be ruled out, and a pattern to the vomiting must be identified