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For examining the effects of each drug treatment on action potential activity, baseline firing rate was sampled and averaged over 1 min immediately prior to drug application, for nicotine, the response magnitude was determined comparing the baseline frequency determination with the average frequency during a 30 sec window centered on the peak effect

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When fed an artificial diet, Manduca larvae do not consume the xanthophyll -which is a yellow pigment- needed to produce their green coloration