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Next, least absolute shrinkage and selection operator (LASSO) Cox regression analysis was conducted utilizing the function glmnet of R (version 4.17) to construct a prognostic model

Protein extracts were separated on 8% SDS-polyacrylamide or 412% bistris protein gel (Invitrogen), transferred on a nitrocellulose membrane, and stained with antibodies against ZO-1 (N1N2, GeneTex, dilution 1:3000)

Ectoin: A natural stress-protection molecule that shields cells from pollution and temperature shifts

Direct NAD is mainly used in clinical or research settings, while NMN and NR are widely available as supplements

B12 injections are ideal for those who have trouble getting enough B12 in their diet