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After incubation, the sections were washed four times in PBS-Triton for 10 min each, followed by incubation with the secondary antibody, Alexa Fluor 488 donkey anti-rabbit IgG (1:200, AntGene, China), for 2 h at room temperature

However, aggregated virions may still attach to some extent to target cells depending upon the nAb and assay conditions, and aggregation may affect a step downstream from viral attachment such as fusion or viral egress from infected cells (see below)

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Zhu YL, et al

Recognizing the likely role that progesterone plays in fibroids and the ability of estrogen to boost progesterone receptor expression, research is now focusing on SPRMs (selective progesterone receptor modulators), sometimes referred to as antiprogestins, that alter PR functionand thus cellular responsivity to progesterone