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There are several chemical, physical, and biological methods of producing NPs, such as laser ablation, coprecipitation, the hydrothermal route, the solvothermal route, the sol-gel process, the polyol process, sonochemistry, and microwave-assisted and electrochemical methods [17]

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Based on nonlinear regression analyses, stress-inducing concentrations corresponding to their 48-h kinetic profiles (10 mM APAP and 20 mM MET) were selected for downstream immune co-cultures to evaluate modulatory effects without inducing absolute target cell ablation (The IC values were previously determined by our team)