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2.7 Immunofluorescence staining After treatment, the cells were washed with pre-cooled PBS, then fixed with 4% paraformaldehyde for 10 minutes, and dropped on a slide to dry

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Conversely, high levels of T 3 and T 4 feedback and decrease the production of TRH

Funding This project was supported by the FoRUM grant F957N2019 of the Ruhr University Bochum, Germany to DL, and the project #57560786 of the German Academic Exchange Service (DAAD) to DL