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The quality and quantity of the extracted DNA were analyzed via 1.8% agarose gel electrophoresis, and the DNA concentration and purity were determined using a NanoDrop 2000 UVVis spectrophotometer (Thermo Scientific, Wilmington, United States)

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When using Hydrocodone Bitartrate and Acetaminophen Tablets with CYP3A4 inducers or discontinuing CYP3A4 inhibitors, follow patients at frequent intervals and consider increasing the opioid dosage if needed to maintain adequate analgesia or if symptoms of opioid withdrawal occur [see PRECAUTIONS