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Description

The following primers were used for PCR: mouse Gapdh , 5-GCCTGGAGAAACCTGCCAAGTATG-3 and 5-GAGTGGGAGTTGCTGTTGAAGTCG-3

In contrast, animals initiated with a low-aflatoxin dose actually produced substantially more foci when subsequently fed the 20% protein diet

Transmission electron microscopy Cells were digested with 0.25% trypsin and 0.02% EDTA, washed with Hanks Balanced Salt Solution (HBSS)

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On one hand, 1,25(OH)D can attenuate VDR ubiquitination and delay its degradation ( Notably, renal transplantation provides direct evidence supporting the reversibility of vitamin D metabolic impairment