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Pathohistological examinations of both acute and chronic toxicity revealed that L-NAME caused severe tissue damage (myocardial and hepatic cell necrosis, and necrosis of the lymphoid cells in the spleen) while L-arginine provoked predominantly congestion, edema and hemorrhages in all organs

Ethics declarations Acknowledgements This work was undertaken as part of UK Biobank project application ref 56653 Pharmacogenomics to improve drug efficacy and safety

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Compared to other cell types in tissues such as spleen, thymus, lymph nodes etc., the DCs have a low density and one of the most commonly Nycodenz-based techniques is simply to isolate lowdensity cells by layering the disaggregated cell suspension over a barrier of density 1.077-1.080 g/ml (e.g

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