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Description

In brief, 5 10 A375R cells were plated in High-Content Imaging Glass Bottom 24-well Microplates for 24 h and then treated with either 3 M R4VPL3-1 for 2.5 h or 100 M Cumene hydroperoxide for 2 h (positive control, an established lipid-peroxidation inducer)

2015;9:BC01BC03

Administered into the muscle for slow, sustained release, these shots take just minutes to perform but deliver powerful benefits that last for days

The plates were briefly stirred and activity was spectrophotometrically determined (SpectraMax ABS Plus, Molecular Devices) at room temperature in kinetic mode at the specific wavelength for each substrate (described above)

Once TGR5 is activated in muscles or brown adipose tissue, it stimulates energy consumption, and in the intestine, it increases the secretion of GLP-1 (Fig