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Has the vial gone through any freeze-thaw cycles

The main trigger of articular bone erosion is synovitis, including the overproduction of ROS and proinflammatory cytokines, as well as RANKL, thereby creating a highly inflammatory and tissue-damaging microenvironment of RA

Growth conditions Strains were grown at 37C in either M9 medium or minimal A medium (Miller, 1972) containing 0.2% glucose, 0.2% casamino acids, 0.5 mM histidine, 0.5 mM tryptophan, 0.5 mM thiamine, 0.01% MgSO 4 , and 0.01% CaCl 2 , unless otherwise noted

doi: 10.1021/jf0634808

For a drug like S/V, it is asking way too much for cardiologists to insist upon awaiting publication of some not-yet-scheduled future, randomised, double-blinded, placebo-controlled, long term, cross-over, peer-reviewed study with hundreds of dogs in each cohort, before being able to prolong the lives of our MVD-affected dogs