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Isolation, Purification, and Structure Elucidation of Unknown-1 Metabolite To identify the chemical structure of the unknown-1 metabolite, multiple incubations of 7-HMG (1 mM) with undiluted human plasma (1 mL) for 24 h at 37 C were performed to obtain enough amount of metabolite unknown-1

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Negative consequences of nutritional habits associated with a Western-style diet may lead to inflammation and production of free radicals (134) through the secretion of numerous pro-inflammatory molecules such as interleukin (IL)- 6, IL-1b, IL-8, and C-reactive protein (CRP), leading to the development of autoimmune disorders either directly, due to inflammation or disturbed immune balance, or indirectly due to increased fat depositions and the development of obesity

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