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Excessive Se intake can lead to adverse health problems and Se toxicity

10.1016/j.freeradbiomed.2016.03.009 Free Radic Biol Med

Improved insight into bacterial copper resistance mechanisms in vivo and in environmental settings will be necessary to optimize antimicrobial uses of copper

We used 1 l 12.5 ng l 1 ccomplementary DNA with intron-spanning primers specific to each gene, and iQ SYBR Green Supermix was used according to the manufacturers instructions (BioRad)