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For the other studies, the mean age maintained heterogeneous ranging from 32 to 72 years of age, yet with a quite balanced distribution based on sex [40,41,43,46]

Western blot Tumor tissues and cells were lysed in RIPA lysis buffer supplemented with protease inhibitors (4693159001, Roche, Basel, Switzerland)

Biomed Pharmacother 91:338349

Complete blood count and biochemical analysis Tubes with anticoagulant (lithium heparin) were sent for biochemical and hematological tests

Proc Natl Acad Sci USA 111:22472252 Chaves C, Remiao F, Cisternino S, Decleves X (2017) Opioids and the blood-brain barrier: a dynamic interaction with consequences on drug disposition in brain