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After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)

Li et al (187) revealed that AS-IV can markedly inhibit ROS release from activated HSCs and mediate the downregulation of lipid hydroperoxide content (188)

This intracellular mechanism differentiates it from peptides that stimulate systemic signaling cascades

2- It is safe to do another blood donation or therapeutic phlebotomy depending on ferritin or iron levels

MRP1 WT-expressing cells experienced a dramatic GSH level drop of about 80% 3 hours upon addition of 20 M verapamil