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Together these results suggest that ORF3a induces mROS production to activate HIF-1, which in turn triggers a shift in cellular metabolism to favor glycolysis, resulting in increased viral replication and transcription of pro-inflammatory cytokines

References Alhowikan AM, Al-Ayadhi LY, Halepoto DM

Analytical challenges in multi-residue citrus fruit extraction arise from individual matrix characteristics

PCR products of candidate clones were further cloned into the pRK5 vector, followed by sequencing to confirm the knockout of RSMC at the single-allele level

Similar approaches have successfully generated cardiac spheroids using rat neonatal ventricular cardiomyocytes, human dermal fibroblasts, and human coronary microartery ECs in ultra-low attachment plates [253], as well as spheroids composed of human iPSC-derived cardiomyocytes, human cardiac fibroblasts, and HUVECs in an ultra-low attachment hanging drop system [254]