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Moreover, both peripheral and CNS related inflammatory pathways have crucial role in the MS pathogenesis

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cDNA synthesis from extracted RNA was performed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA) on the Veriti 96-Well Thermal Cycler platform (Applied Biosystems, Foster City, CA, USA), maintaining a 1:1 ratio of reverse transcriptase buffer and RNA

COMBAT) but should still be kept in consideration

This process is carefully controlled to ensure the purity and quality of the final product