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Burgos-Barragan G, Wit N, Meiser J, Dingler FA, Pietzke M, Mulderrig L, et al

In brief, bones were fully decalcified in 14% EDTA at a pH of 7.4 for 2 weeks before embedding in optimal cutting temperature mounting media (Fisher HealthCare, 23-730-571) and sectioning at 50 m on a cryostat (Leica)

One unique feature was that the physiological separation between estrogen- and progesterone-induced changes, in which progesterone downregulation of ERs prevent co-stimulation of both pathways, was perturbed in the PRM-exposed patients

In a dehydrated state, the liver works less effectively, and the elimination of alcohol from the body is delayed