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After High glucose, Erastin and Ferr-1 intervention were used to treat the cells, the cells were incubated with the CCK-8 solution for 2 h, and then the cell viability was measured at the absorbance of 450 nm using a microplate reader (Molecular Devices, Sunnyvale, CA, USA)

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This is why it is a good idea to use another product on top of this one to help "seal in" the moisture (look for ingredients like dimethicone, squalane, oils and butters)

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