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HallstromSFranzMGasserHVodrazkaMSemsrothSLosertUMet al

The compositions of the fatty acids and alkyl substituents of phosphatidylcholines are listed in Table 2

[PubMed] [Google Scholar] 26.Habib L

Additionally, we identify a particular cluster of intratumoral CD8 + T cells expressing a putative gene signature of GSH metabolism (GMGS) in association with clinical response and survival across several human cancers

KEAP1 mass spectrometry analysis For mass spectrometry analysis, 10 g of recombinant human KEAP1 (#11981-H29B-100, Sino Biologicals, Beijin, PR China) were diluted to 100 l with 20 mM Tris pH 8.0 and diafiltrated by means of a Amicon Ultra-0.5 ml device (Sigma-Aldrich, Milan, Italy) to eliminate GSH, and then treated with 30 mM M DTT and incubated 30 min at room temperature to reduce the disulfide bridges, diafiltrated to remove the excess of DTT and diluted in 20 mM Tris pH 8.0