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A rotatory microtome was used to cut 5 m slices of each sample, which were subsequently placed on coated glass slides for H&E staining 49 or positive glass slides for immunohistochemical staining of P53 and caspase-3.The immune histochemistry protocol was followed according to the protocol of Petrosyan, et al

Whole-blood measurements are dominated by erythrocyte glutathione and require appropriate volume- or erythrocyte-based normalization, whereas plasma and serum contain much lower concentrations and are particularly sensitive to hemolysis and processing delay

Vukojevi, J., Milavi, M., Perovi, D., Ili, S., ili, A

PMID: 26451969