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After incubation for 5 h, 2.4 M Cas9 protein, 3.7 M crRNA, 7.4 M tracrRNA, and 100 ng/l ssODN were electroporated into zygotes by using a NEPA 21 electroporator (NEPAGENE) 57

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Research-Use Dosing Conventions (Not a Recommendation) This section describes the dose ranges used inside clinical trials and laboratory research settings only