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After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)

The results revealed that 14.1% of participants met the criteria for FM, a finding consistent with those of some international and regional studies, which have reported prevalence rates ranging from 2% to 17% depending on the population and diagnostic criteria used [4, 11]

If IBD, EPI, SIBO, or parasites are driving the poor absorption, those conditions need their own treatment plan running alongside B12 therapy

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