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Beta-oxidation of these fatty acids in mitochondria generates reactive oxygen species (ROS) as a normal byproduct

More recent work has used HepG2, a human hepatoblastoma cell line [99], and CRISPR/Cas9 gene editing to create an SLC25A13 knockout via targeted deletion of a ~43 kb region spanning exons 3 and 4 in one allele, with a serendipitous 10 bp deletion in the second allele [53] (Figure 6)