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Supportive peptides such as oral BPC157 or KPV to repair mucosal integrity

11, the ELISA reader used for testing needs to be installed with the ability to detect 45010nmWavelength filters with optical density range of 0-3.5It is recommended to use in advance 15Minutes to preheat

The thiolated poly (ethylene glycol) folate (SH-PEG 5000 -FA) was obtained by coupling SH-PEG-NH 2 (Mn 5,000) with Folate N-hydroxysuccinimidyl ester via a previously published method [29-31]

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4, remove the sealing film, discard the liquid, pat dry on absorbent paper, fill each well with washing solution, and let it stand1min, shake off the detergent, pat dry on absorbent paper, and repeat this process.5If you use an automatic plate washer, please follow the plate washer operating procedures to wash the plate and add soaking30sAfter washing the plate, pat the plate dry on a clean, lint-free paper before adding the substrate