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Cattle disperse seeds in two common ways: Dispersal through dung Cattle disperse tree seeds through dung deposits, this mostly occurs in trees with hard seed shells or that require seed dormancy treatments , they mostly have large woody protective outer coatings

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The resulting clots were rinsed with PBS at least 3 times and dehydrated in an ascending alcohol series (50%, 70%, 90%, and 100%) and dried in a CO 2 critical point drying apparatus