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The membrane pellet was homogenized and solubilized by gentle nutation for 2 hours at 4 C in buffer consisting of 20 mM Tris-Cl (pH 8.0), 150 mM NaCl, 1% Dodecyl--D-Maltosid (w/v, DDM), and 20 M DNQX, followed by centrifugation at 200,000 g for 30 min to remove insoluble material

A more comprehensive approach would be to concurrently measure actual protein levels (e.g., WB, ELISA, etc.) of potential targets in addition to inhibitor assays

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Long-term metabolic benefits of exenatide in mice are mediated solely via the known glucagon-like peptide 1 receptor