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FLAG-tagged FUS/TLS and HA-tagged SMN were expressed in HeLa cells using DNA transfection, extracts were prepared, FUS/TLS-containing complexes were recovered with FLAG antibody and finally any co-purified SMN was detected with an HA antibody

Neural stem cells: developmental mechanisms and disease modeling

Abstract Affinity chromatography is the method of choice for the rapid purification of proteins from cell extracts or culture supernatants

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PLoS Pathog (2016) 12:e1005480.10.1371/journal.ppat.1005480 187 WhelanRARauschSEbnerFGunzelDRichterJFHeringNAet alA transgenic probiotic secreting a parasite immunomodulator for site-directed treatment of gut inflammation